cat 6057 gmp recombinant human sonic hedgehog Search Results


recombinant human noggin  (R&D Systems)
96
R&D Systems recombinant human noggin
Figure 1. Differentiation of human pluripotent stem cells to neuroepithelium under defined conditions. (A): Experimental timeline. (B): Reverse transcriptase polymerase chain reaction analysis of pluripotency, mesoderm, endoderm, and neuroectoderm gene expres- sion in differentiating H9 human embryonic stem cells (hESCs). “SB” indicates addition of SB431542 and “N” indicates addition of nog- gin. (C): Flow cytometry analysis of Pax6. Data are presented as mean 6 SD calculated from at least two biological replicates. Differentiation was conducted on Matrigel-coated substrates unless otherwise specified. (D): Images of neural rosette formation on day 6 of H9 hESC differentiation. The inset shows the magnified rosette structure. All images are of cells differentiated on Matrigel- coated substrates except for the one labeled “E6 (VTN-NC),” which indicates cells differentiated in E6 medium on substrates coated with <t>recombinant</t> vitronectin peptide. Scale bars in Pax6/N-cadherin-stained images are 250 mm; scale bars in Otx2/Sox2-stained images are 50 mm. (E): Representative flow cytometry histograms of N-cadherin, Otx2, and Sox2 expression at day 6 of differentiating H9 hESCs in E6 medium. Data are representative of two biological replicates and mean 6 SD are listed in Results. Gray histogram, IgG control; red histogram, label of interest. (F): Progression of Sox1 expression in E6/FI conditions with and without retinoic acid. Scale bars 5 100 mm.
Recombinant Human Noggin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant human noggin/product/R&D Systems
Average 96 stars, based on 1 article reviews
recombinant human noggin - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
recombinant human noggin protein, cf  (Bio-Techne corporation)
94
Bio-Techne corporation recombinant human noggin protein, cf
Figure 1. Differentiation of human pluripotent stem cells to neuroepithelium under defined conditions. (A): Experimental timeline. (B): Reverse transcriptase polymerase chain reaction analysis of pluripotency, mesoderm, endoderm, and neuroectoderm gene expres- sion in differentiating H9 human embryonic stem cells (hESCs). “SB” indicates addition of SB431542 and “N” indicates addition of nog- gin. (C): Flow cytometry analysis of Pax6. Data are presented as mean 6 SD calculated from at least two biological replicates. Differentiation was conducted on Matrigel-coated substrates unless otherwise specified. (D): Images of neural rosette formation on day 6 of H9 hESC differentiation. The inset shows the magnified rosette structure. All images are of cells differentiated on Matrigel- coated substrates except for the one labeled “E6 (VTN-NC),” which indicates cells differentiated in E6 medium on substrates coated with <t>recombinant</t> vitronectin peptide. Scale bars in Pax6/N-cadherin-stained images are 250 mm; scale bars in Otx2/Sox2-stained images are 50 mm. (E): Representative flow cytometry histograms of N-cadherin, Otx2, and Sox2 expression at day 6 of differentiating H9 hESCs in E6 medium. Data are representative of two biological replicates and mean 6 SD are listed in Results. Gray histogram, IgG control; red histogram, label of interest. (F): Progression of Sox1 expression in E6/FI conditions with and without retinoic acid. Scale bars 5 100 mm.
Recombinant Human Noggin Protein, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant human noggin protein, cf/product/Bio-Techne corporation
Average 94 stars, based on 1 article reviews
recombinant human noggin protein, cf - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
penicillin streptomycin  (R&D Systems)
93
R&D Systems penicillin streptomycin
Figure 1. Differentiation of human pluripotent stem cells to neuroepithelium under defined conditions. (A): Experimental timeline. (B): Reverse transcriptase polymerase chain reaction analysis of pluripotency, mesoderm, endoderm, and neuroectoderm gene expres- sion in differentiating H9 human embryonic stem cells (hESCs). “SB” indicates addition of SB431542 and “N” indicates addition of nog- gin. (C): Flow cytometry analysis of Pax6. Data are presented as mean 6 SD calculated from at least two biological replicates. Differentiation was conducted on Matrigel-coated substrates unless otherwise specified. (D): Images of neural rosette formation on day 6 of H9 hESC differentiation. The inset shows the magnified rosette structure. All images are of cells differentiated on Matrigel- coated substrates except for the one labeled “E6 (VTN-NC),” which indicates cells differentiated in E6 medium on substrates coated with <t>recombinant</t> vitronectin peptide. Scale bars in Pax6/N-cadherin-stained images are 250 mm; scale bars in Otx2/Sox2-stained images are 50 mm. (E): Representative flow cytometry histograms of N-cadherin, Otx2, and Sox2 expression at day 6 of differentiating H9 hESCs in E6 medium. Data are representative of two biological replicates and mean 6 SD are listed in Results. Gray histogram, IgG control; red histogram, label of interest. (F): Progression of Sox1 expression in E6/FI conditions with and without retinoic acid. Scale bars 5 100 mm.
Penicillin Streptomycin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/penicillin streptomycin/product/R&D Systems
Average 93 stars, based on 1 article reviews
penicillin streptomycin - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
gmp r d systems  (R&D Systems)
94
R&D Systems gmp r d systems
Figure 1. Differentiation of human pluripotent stem cells to neuroepithelium under defined conditions. (A): Experimental timeline. (B): Reverse transcriptase polymerase chain reaction analysis of pluripotency, mesoderm, endoderm, and neuroectoderm gene expres- sion in differentiating H9 human embryonic stem cells (hESCs). “SB” indicates addition of SB431542 and “N” indicates addition of nog- gin. (C): Flow cytometry analysis of Pax6. Data are presented as mean 6 SD calculated from at least two biological replicates. Differentiation was conducted on Matrigel-coated substrates unless otherwise specified. (D): Images of neural rosette formation on day 6 of H9 hESC differentiation. The inset shows the magnified rosette structure. All images are of cells differentiated on Matrigel- coated substrates except for the one labeled “E6 (VTN-NC),” which indicates cells differentiated in E6 medium on substrates coated with <t>recombinant</t> vitronectin peptide. Scale bars in Pax6/N-cadherin-stained images are 250 mm; scale bars in Otx2/Sox2-stained images are 50 mm. (E): Representative flow cytometry histograms of N-cadherin, Otx2, and Sox2 expression at day 6 of differentiating H9 hESCs in E6 medium. Data are representative of two biological replicates and mean 6 SD are listed in Results. Gray histogram, IgG control; red histogram, label of interest. (F): Progression of Sox1 expression in E6/FI conditions with and without retinoic acid. Scale bars 5 100 mm.
Gmp R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gmp r d systems/product/R&D Systems
Average 94 stars, based on 1 article reviews
gmp r d systems - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
y27632 rocki tocris 1254 recombinant human noggin bio techne 6057 ng 025 xav939 sigma aldrich x3004  (ZeptoMetrix corporation)
92
ZeptoMetrix corporation y27632 rocki tocris 1254 recombinant human noggin bio techne 6057 ng 025 xav939 sigma aldrich x3004
Figure 1. Differentiation of human pluripotent stem cells to neuroepithelium under defined conditions. (A): Experimental timeline. (B): Reverse transcriptase polymerase chain reaction analysis of pluripotency, mesoderm, endoderm, and neuroectoderm gene expres- sion in differentiating H9 human embryonic stem cells (hESCs). “SB” indicates addition of SB431542 and “N” indicates addition of nog- gin. (C): Flow cytometry analysis of Pax6. Data are presented as mean 6 SD calculated from at least two biological replicates. Differentiation was conducted on Matrigel-coated substrates unless otherwise specified. (D): Images of neural rosette formation on day 6 of H9 hESC differentiation. The inset shows the magnified rosette structure. All images are of cells differentiated on Matrigel- coated substrates except for the one labeled “E6 (VTN-NC),” which indicates cells differentiated in E6 medium on substrates coated with <t>recombinant</t> vitronectin peptide. Scale bars in Pax6/N-cadherin-stained images are 250 mm; scale bars in Otx2/Sox2-stained images are 50 mm. (E): Representative flow cytometry histograms of N-cadherin, Otx2, and Sox2 expression at day 6 of differentiating H9 hESCs in E6 medium. Data are representative of two biological replicates and mean 6 SD are listed in Results. Gray histogram, IgG control; red histogram, label of interest. (F): Progression of Sox1 expression in E6/FI conditions with and without retinoic acid. Scale bars 5 100 mm.
Y27632 Rocki Tocris 1254 Recombinant Human Noggin Bio Techne 6057 Ng 025 Xav939 Sigma Aldrich X3004, supplied by ZeptoMetrix corporation, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/y27632 rocki tocris 1254 recombinant human noggin bio techne 6057 ng 025 xav939 sigma aldrich x3004/product/ZeptoMetrix corporation
Average 92 stars, based on 1 article reviews
y27632 rocki tocris 1254 recombinant human noggin bio techne 6057 ng 025 xav939 sigma aldrich x3004 - by Bioz Stars, 2026-02
92/100 stars
  Buy from Supplier
noggin  (R&D Systems)
96
R&D Systems noggin
Figure 1. Differentiation of human pluripotent stem cells to neuroepithelium under defined conditions. (A): Experimental timeline. (B): Reverse transcriptase polymerase chain reaction analysis of pluripotency, mesoderm, endoderm, and neuroectoderm gene expres- sion in differentiating H9 human embryonic stem cells (hESCs). “SB” indicates addition of SB431542 and “N” indicates addition of nog- gin. (C): Flow cytometry analysis of Pax6. Data are presented as mean 6 SD calculated from at least two biological replicates. Differentiation was conducted on Matrigel-coated substrates unless otherwise specified. (D): Images of neural rosette formation on day 6 of H9 hESC differentiation. The inset shows the magnified rosette structure. All images are of cells differentiated on Matrigel- coated substrates except for the one labeled “E6 (VTN-NC),” which indicates cells differentiated in E6 medium on substrates coated with <t>recombinant</t> vitronectin peptide. Scale bars in Pax6/N-cadherin-stained images are 250 mm; scale bars in Otx2/Sox2-stained images are 50 mm. (E): Representative flow cytometry histograms of N-cadherin, Otx2, and Sox2 expression at day 6 of differentiating H9 hESCs in E6 medium. Data are representative of two biological replicates and mean 6 SD are listed in Results. Gray histogram, IgG control; red histogram, label of interest. (F): Progression of Sox1 expression in E6/FI conditions with and without retinoic acid. Scale bars 5 100 mm.
Noggin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/noggin/product/R&D Systems
Average 96 stars, based on 1 article reviews
noggin - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
human noggin  (R&D Systems)
93
R&D Systems human noggin
Figure 1. Differentiation of human pluripotent stem cells to neuroepithelium under defined conditions. (A): Experimental timeline. (B): Reverse transcriptase polymerase chain reaction analysis of pluripotency, mesoderm, endoderm, and neuroectoderm gene expres- sion in differentiating H9 human embryonic stem cells (hESCs). “SB” indicates addition of SB431542 and “N” indicates addition of nog- gin. (C): Flow cytometry analysis of Pax6. Data are presented as mean 6 SD calculated from at least two biological replicates. Differentiation was conducted on Matrigel-coated substrates unless otherwise specified. (D): Images of neural rosette formation on day 6 of H9 hESC differentiation. The inset shows the magnified rosette structure. All images are of cells differentiated on Matrigel- coated substrates except for the one labeled “E6 (VTN-NC),” which indicates cells differentiated in E6 medium on substrates coated with <t>recombinant</t> vitronectin peptide. Scale bars in Pax6/N-cadherin-stained images are 250 mm; scale bars in Otx2/Sox2-stained images are 50 mm. (E): Representative flow cytometry histograms of N-cadherin, Otx2, and Sox2 expression at day 6 of differentiating H9 hESCs in E6 medium. Data are representative of two biological replicates and mean 6 SD are listed in Results. Gray histogram, IgG control; red histogram, label of interest. (F): Progression of Sox1 expression in E6/FI conditions with and without retinoic acid. Scale bars 5 100 mm.
Human Noggin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human noggin/product/R&D Systems
Average 93 stars, based on 1 article reviews
human noggin - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
recombinant human noggin gmp protein  (R&D Systems)
93
R&D Systems recombinant human noggin gmp protein
Figure 1. Differentiation of human pluripotent stem cells to neuroepithelium under defined conditions. (A): Experimental timeline. (B): Reverse transcriptase polymerase chain reaction analysis of pluripotency, mesoderm, endoderm, and neuroectoderm gene expres- sion in differentiating H9 human embryonic stem cells (hESCs). “SB” indicates addition of SB431542 and “N” indicates addition of nog- gin. (C): Flow cytometry analysis of Pax6. Data are presented as mean 6 SD calculated from at least two biological replicates. Differentiation was conducted on Matrigel-coated substrates unless otherwise specified. (D): Images of neural rosette formation on day 6 of H9 hESC differentiation. The inset shows the magnified rosette structure. All images are of cells differentiated on Matrigel- coated substrates except for the one labeled “E6 (VTN-NC),” which indicates cells differentiated in E6 medium on substrates coated with <t>recombinant</t> vitronectin peptide. Scale bars in Pax6/N-cadherin-stained images are 250 mm; scale bars in Otx2/Sox2-stained images are 50 mm. (E): Representative flow cytometry histograms of N-cadherin, Otx2, and Sox2 expression at day 6 of differentiating H9 hESCs in E6 medium. Data are representative of two biological replicates and mean 6 SD are listed in Results. Gray histogram, IgG control; red histogram, label of interest. (F): Progression of Sox1 expression in E6/FI conditions with and without retinoic acid. Scale bars 5 100 mm.
Recombinant Human Noggin Gmp Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant human noggin gmp protein/product/R&D Systems
Average 93 stars, based on 1 article reviews
recombinant human noggin gmp protein - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
house n a recombinant human noggin r d 6057 ng  (WuXi Biologics)
86
WuXi Biologics house n a recombinant human noggin r d 6057 ng
Figure 1. Differentiation of human pluripotent stem cells to neuroepithelium under defined conditions. (A): Experimental timeline. (B): Reverse transcriptase polymerase chain reaction analysis of pluripotency, mesoderm, endoderm, and neuroectoderm gene expres- sion in differentiating H9 human embryonic stem cells (hESCs). “SB” indicates addition of SB431542 and “N” indicates addition of nog- gin. (C): Flow cytometry analysis of Pax6. Data are presented as mean 6 SD calculated from at least two biological replicates. Differentiation was conducted on Matrigel-coated substrates unless otherwise specified. (D): Images of neural rosette formation on day 6 of H9 hESC differentiation. The inset shows the magnified rosette structure. All images are of cells differentiated on Matrigel- coated substrates except for the one labeled “E6 (VTN-NC),” which indicates cells differentiated in E6 medium on substrates coated with <t>recombinant</t> vitronectin peptide. Scale bars in Pax6/N-cadherin-stained images are 250 mm; scale bars in Otx2/Sox2-stained images are 50 mm. (E): Representative flow cytometry histograms of N-cadherin, Otx2, and Sox2 expression at day 6 of differentiating H9 hESCs in E6 medium. Data are representative of two biological replicates and mean 6 SD are listed in Results. Gray histogram, IgG control; red histogram, label of interest. (F): Progression of Sox1 expression in E6/FI conditions with and without retinoic acid. Scale bars 5 100 mm.
House N A Recombinant Human Noggin R D 6057 Ng, supplied by WuXi Biologics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/house n a recombinant human noggin r d 6057 ng/product/WuXi Biologics
Average 86 stars, based on 1 article reviews
house n a recombinant human noggin r d 6057 ng - by Bioz Stars, 2026-02
86/100 stars
  Buy from Supplier
recombinant murine noggin  (R&D Systems)
90
R&D Systems recombinant murine noggin
Figure 1. Differentiation of human pluripotent stem cells to neuroepithelium under defined conditions. (A): Experimental timeline. (B): Reverse transcriptase polymerase chain reaction analysis of pluripotency, mesoderm, endoderm, and neuroectoderm gene expres- sion in differentiating H9 human embryonic stem cells (hESCs). “SB” indicates addition of SB431542 and “N” indicates addition of nog- gin. (C): Flow cytometry analysis of Pax6. Data are presented as mean 6 SD calculated from at least two biological replicates. Differentiation was conducted on Matrigel-coated substrates unless otherwise specified. (D): Images of neural rosette formation on day 6 of H9 hESC differentiation. The inset shows the magnified rosette structure. All images are of cells differentiated on Matrigel- coated substrates except for the one labeled “E6 (VTN-NC),” which indicates cells differentiated in E6 medium on substrates coated with <t>recombinant</t> vitronectin peptide. Scale bars in Pax6/N-cadherin-stained images are 250 mm; scale bars in Otx2/Sox2-stained images are 50 mm. (E): Representative flow cytometry histograms of N-cadherin, Otx2, and Sox2 expression at day 6 of differentiating H9 hESCs in E6 medium. Data are representative of two biological replicates and mean 6 SD are listed in Results. Gray histogram, IgG control; red histogram, label of interest. (F): Progression of Sox1 expression in E6/FI conditions with and without retinoic acid. Scale bars 5 100 mm.
Recombinant Murine Noggin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant murine noggin/product/R&D Systems
Average 90 stars, based on 1 article reviews
recombinant murine noggin - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


Figure 1. Differentiation of human pluripotent stem cells to neuroepithelium under defined conditions. (A): Experimental timeline. (B): Reverse transcriptase polymerase chain reaction analysis of pluripotency, mesoderm, endoderm, and neuroectoderm gene expres- sion in differentiating H9 human embryonic stem cells (hESCs). “SB” indicates addition of SB431542 and “N” indicates addition of nog- gin. (C): Flow cytometry analysis of Pax6. Data are presented as mean 6 SD calculated from at least two biological replicates. Differentiation was conducted on Matrigel-coated substrates unless otherwise specified. (D): Images of neural rosette formation on day 6 of H9 hESC differentiation. The inset shows the magnified rosette structure. All images are of cells differentiated on Matrigel- coated substrates except for the one labeled “E6 (VTN-NC),” which indicates cells differentiated in E6 medium on substrates coated with recombinant vitronectin peptide. Scale bars in Pax6/N-cadherin-stained images are 250 mm; scale bars in Otx2/Sox2-stained images are 50 mm. (E): Representative flow cytometry histograms of N-cadherin, Otx2, and Sox2 expression at day 6 of differentiating H9 hESCs in E6 medium. Data are representative of two biological replicates and mean 6 SD are listed in Results. Gray histogram, IgG control; red histogram, label of interest. (F): Progression of Sox1 expression in E6/FI conditions with and without retinoic acid. Scale bars 5 100 mm.

Journal: Stem cells (Dayton, Ohio)

Article Title: Defined human pluripotent stem cell culture enables highly efficient neuroepithelium derivation without small molecule inhibitors.

doi: 10.1002/stem.1622

Figure Lengend Snippet: Figure 1. Differentiation of human pluripotent stem cells to neuroepithelium under defined conditions. (A): Experimental timeline. (B): Reverse transcriptase polymerase chain reaction analysis of pluripotency, mesoderm, endoderm, and neuroectoderm gene expres- sion in differentiating H9 human embryonic stem cells (hESCs). “SB” indicates addition of SB431542 and “N” indicates addition of nog- gin. (C): Flow cytometry analysis of Pax6. Data are presented as mean 6 SD calculated from at least two biological replicates. Differentiation was conducted on Matrigel-coated substrates unless otherwise specified. (D): Images of neural rosette formation on day 6 of H9 hESC differentiation. The inset shows the magnified rosette structure. All images are of cells differentiated on Matrigel- coated substrates except for the one labeled “E6 (VTN-NC),” which indicates cells differentiated in E6 medium on substrates coated with recombinant vitronectin peptide. Scale bars in Pax6/N-cadherin-stained images are 250 mm; scale bars in Otx2/Sox2-stained images are 50 mm. (E): Representative flow cytometry histograms of N-cadherin, Otx2, and Sox2 expression at day 6 of differentiating H9 hESCs in E6 medium. Data are representative of two biological replicates and mean 6 SD are listed in Results. Gray histogram, IgG control; red histogram, label of interest. (F): Progression of Sox1 expression in E6/FI conditions with and without retinoic acid. Scale bars 5 100 mm.

Article Snippet: The following morning, cells were changed to E6 medium, E6 containing 10 mM SB431542 (Cellagentech, San Diego, CA (www.cellagen tech.com)), or E6 containing 10 mM SB431542 and 200 ng/ml recombinant human noggin (R&D Systems) to initiate differentiation.

Techniques: Reverse Transcription, Polymerase Chain Reaction, Flow Cytometry, Labeling, Recombinant, Staining, Cytometry, Expressing, Control